如果您无法下载资料，请参考说明：
1、部分资料下载需要金币，请确保您的账户上有足够的金币
2、已购买过的文档，再次下载不重复扣费
3、资料包下载后请先用软件解压，在使用对应软件打开
会计学Keywordsscutellarin;scutellarein;UGT;enzymeinhibition.Contents1.IntroductionIntherecentyears,anotherimportantdrugmetabolizingenzymeUDP-glucuronosyltransferase(UGT)hasbeendemonstratedtoexhibitsignificantcontributiontowardsthemetabolismofclinicaldrugsandherbalcomponents,andmoreandmoreattentionhasbeengiventothisenzymes(MalikandBlack,2012;Lietal.,2012).UGTshavebeendemonstratedtobeinvolvedinthemetaboliceliminationofmanyimportantendogenoussubstances,suchasbilirubin,bileacidandestradiol(Erichsenetal.,2010).Guoetal.showeddeglycosylationprocessofliquiritinstronglyenhancedtheinhibitorycapabilitytowardsUGTs(2012).Huangetal.showedstronginhibitionofglycyrrhetinicacidtowardsUGT1A3and2B7(2012).Liuetal.alsousedinvitroincubationsystemtofindthestronginhibitionofdeoxyschizandrinandschisantherinAtowardsUGT1A3(2012).Chemicalsandreagents.Scutellarin,Scutellarein,4-MU,Tris-HCl,7-hydroxycoumarin,UDPGA(缓冲(huǎnchōng)盐)，重组UGTs亚型（UGT1A1，UGT1A6，UGT1A9，UGT2B7），HPLCreagents.Incubationandanalysismethodsforinhibitionevaluation.After5minpre-incubationat37℃,theUDPGAwasaddedinthemixturetoinitiatethereaction.Theincubationtimewas120minforUGT1A1andUGT2B7,30min,orUGT1A6andUGT1A9,respectively.Thereactionswereterminatedbyadding100mLacetonitrilewith7-hydroxycoumarin(100mM)asinternalstandard.Themixturewascentrifugedat20000×gfor10min,HPLCanalysis.Datafittingfordeterminationofinhibitiontypeandparameters(Ki).Thereactionvelocitywasdeterminedusingvariousconcentrationsof4-MUandscutellarein.ThedatawerefittedusingDixonplotandLineweaver–Burkdoublereciprocalplot.ThesecondplotwiththeslopesfromtheLineweaver–BurkplotversustheconcentrationsofscutellareinwasutilizedtocalculatetheKivalue.Predictionofinvivodrug–druginteractionmagnitude.Predictionofinvivodrug–druginteractionmagnitude.Themostcommonequationfordrug–druginteractionpredictionwasemployedtopredicttheAUCalterationofcoadministereddrugscausedbyco-administrationofscutellarein.3.Resultsanddiscussion图2表明，100mM野黄芩(huánɡqín)苷对UGT1A1，UGT1A6，UGT1A9，UGT2
