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BiosensorsandBioelectronics26(2011)3371–3375ContentslistsavailableatScienceDirectBiosensorsandBioelectronicsjournalhomepage:www.elsevier.com/locate/biosShortcommunicationElectrochemicalcell-basedchipforthedetectionoftoxiceffectsofbisphenol-AonneuroblastomacellsMd.AbdulKafia,Tae-HyungKimb,JeungHeeAnb,Jeong-WooChoia,b,∗aInterdisciplinaryProgramofIntegratedBiotechnology,SogangUniversity,#1Shinsu-Dong,Mapo-Gu,Seoul121-742,RepublicofKoreabDepartmentofChemical&BiomolecularEngineering,SogangUniversity,#1Shinsu-Dong,Mapo-Gu,Seoul121-742,RepublicofKoreaarticleinfoabstractArticlehistory:Acell-basedchipwasfabricatedfortheelectrochemicaldetectionofthedose-dependenteffectsofReceived27October2010bisphenol-A(BPA)onneuroblastomacells(SH-SY5Y),whichshoweddual-modecorrelationasastandardReceivedinrevisedform2December2010curve.ToxicityassessmentofBPAbecameveryimportantinenvironmentaltoxicantsdetectionsinceBPAAccepted16December2010canbereachedouteasilyfromvariouscommonplastic-basedproductandgivenegativecellulareffectsAvailableonline23December2010onlivingorganism.CellchipwasfabricatedbyimmobilizingcellsonC(RGD)4peptidecoatedelectrodetodetectthecytotoxicityofBPAelectrochemically.RedoxpropertiesinlivingcellsweredeterminedbyKeywords:cyclicvoltammetryusingahome-madethree-electrodesystem,andthecathodicpeakcurrent(I)wasBisphenol-ApcCyclicvoltammetryusedasaparameterformeasurementoftheeffectofBPAoncellviability.Thepeakcurrent,IpcvalueMTTassayincreasedwiththeconcentrationofBPAupto300nMandthendecreasedbecauseofthestimulationofSH-SY5YcellscancercellactivityattheconcentrationofBPAbelow300nMandcytotoxicityattheconcentrationofBPANanobiochipabove300nM,respectively.MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide)assayandopticalmicroscopy-basedmorphologicalanalysisconfirmedtheresultsofelectrochemicalstudy.Thisdual-modecorrelationbetweentheconcentrationofBPAandvoltammetricsignalintensityshouldbefirstlyconsideredtoanalyzeitsdose-dependentstimulusandcytotoxic
