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LysisbufferforEscherichiacoli(BL21):Prepare1MstocksolutionsofKH2PO4andK2HPO4.For100ml,dissolvethefollowingreagentsin90mlofdeionizedwater:0.3mlKH2PO44.7mlK2HPO42.3gNaCl0.75gKCl10mlglycerol0.5mlTritonX-10068mgimidazoleMixthoroughlyandadjustpHto7.8withHCl.Bringthevolumeto100ml.Storeat+4°C.1.5MTris-HCl,pH8.818.17gTrismabasein100mlDWandadjustpHwithHCl1MTris-HCl,pH6.812.11gTrismabasein100mlDWadjustpHwithHCl10%SDS10g/100mlDWTEMED10%Ammoniumpersulfate(APS)Tris-glycineelectrophoresisbuffer(1Lof10Xbuffer)30gofTris-base145gofglycine10gofSDSAworkingsolution,dilute100mLof10Xstockwith900mLofdistilledH2O.NopHadjustmentisnecessary.Tris-glycinetransferbuffer(1Lof10Xbuffer)30gofTris-base145gofglycineAworkingsolution,add100mLof10Xstockto700mLofdistilledH2Oand200mLofmethanol.NopHadjustmentisnecessary.2XSDS-PAGESampleBuffer1.Combinethefollowingreagents:0.5MTris-HCl,pH6.82.5ml,(1.0MTris-HCl,pH6.81.25ml)Glycerol(100%)2.0mlβ-mercaptoethanol0.4mlBromophenolBlue0.02gSDS0.4g2.Bringthevolumeto10mlwithsterilewater.3.Aliquotandfreezeat-20°Cuntilneeded.5xSDS-PAGEsamplebuffer:0.3125MTris·Cl,pH6.8,(1.0MTris-HCl,pH6.86.25ml)50%glycerol(10ml)10%SDS(2g)0.1%bromophenolblue(0.02g)0.25MDTT(or5%β-mercaptoethanol)(1ml)Totalvolume:20mlAliquotandfreezeat-20°Cuntilneeded.SDS-PAGEsamplebuffers（Qiaexpressionist）2xSDS-PAGEsamplebuffer:0.09MTris·Cl,pH6.8;20%glycerol;2%SDS;0.02%bromophenolblue;0.1MDTT5xSDS-PAGEsamplebuffer:0.225MTris·Cl,pH6.8;50%glycerol;5%SDS;0.05%bromophenolblue;0.25MDTT
