PAGE9PAGE\*MERGEFORMAT10Wnt4重组蛋白对鸡等级卵泡颗粒细胞相关基因表达的影响摘要鸡的卵泡选择与产蛋量高度相关，研究卵泡选择的分子机制可为提高其产蛋性能提供一定的理论依据。Wnt信号通路能够调控很多卵巢生长发育过程中的生理过程，如卵泡发育、排卵和黄体化。Wnt4是Wnt家族的成员，对雌性动物的生殖系统起到一定的调控作用。为了研究Wnt4对鸡卵泡选择的调控作用，本研究检测了Wnt4重组蛋白对鸡等级卵泡颗粒细胞中相关基因表达的影响作用，结果发现Wnt4重组蛋白能够显著上调FSHR和CTNNB1的表达，并对StAR和CYP11A1的表达有一定的促进作用。本研究的结果可为深入研究Wnt4的具体功能提供一定的研究基础，也可为进一步阐明鸡卵泡选择的分子机制提供参考。关键词：鸡；卵泡选择；Wnt信号通路；Wnt4；qRT-PCREffectofWnt4RecombinantProteinonExpressionofGenesRelatedtoGranulosaCellsofChickenhierarchalFollicleABSTRACTOvarianfollicleselectionisanimportantprocessimpactingthefecundityofhens.Studyingthemolecularmechanismoffollicleselectioncanprovideatheoreticalbasisforimprovingeggproductionperformance.Wntsignalingpathwaycanregulatemanyphysiologicalprocessesduringovariangrowthanddevelopment,suchasfollicledevelopment,ovulationandluteinization.Wnt4isamemberoftheWntfamily,itplaysaregulatoryroleinthereproductivesystemoffemaleanimals.InordertostudytheeffectofWnt4onfollicleselection,thisstudyexaminedtheeffectofWnt4recombinantproteinontheexpressionofrelatedgenesinchickenhierarchalfolliclegranulosacells.TheresultsshowedthatWnt4recombinantproteincouldsignificantlyincreasedtheexpressionofFSHRandCTNNB1,italsocouldpromotetheexpressionofStARandCYP11A1.TheresultsofthisstudycanprovideacertainresearchbasisforthestudyofthefunctionofWnt4,andalsoprovideareferenceforfurtherelucidatingthemolecularmechanismofchickenfollicleselection.Keywords：Chicken;follicleselection;Wntsignalingpathway;Wnt4;qRT-PCR目录目录HYPERLINK\l_Toc26743_WPSOffice_Level11.文献综述1HYPERLINK\l_Toc5576_WPSOffice_Level21.1鸡的繁殖特点1HYPERLINK\l_Toc16869_WPSOffice_Level31.1.1卵泡发育1HYPERLINK\l_Toc17553_WPSOffice_Level31.1.2卵泡选择1HYPERLINK\l_Toc32243_WPSOffice_Level21.2Wnt信号通路2HYPERLINK\l_Toc19015_WPSOffice_Level31.2.1Wnt信号通路简介2HYPERLINK\l_Toc1487_WPSOffice_Level31.2.2Wnt家族成员在卵泡发育中的作用2HYPERLINK\l_Toc5088_WPSOffice_Level21.3Wnt42HYPERLINK\l_Toc293_WPSOffice_Level31.3.1Wnt4简介2HYPERLINK